Protein ladders and standards for SDS-PAGE, western blots, and isoelectric focusing (IEF)
Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. Each protein standard or ladder is supplied in a ready-to-use format, eliminating the need to reduce, pre-mix, or add loading dyes. These protein standards and ladders are consistent from lot to lot and are strictly quality controlled on appropriate gels to help ensure consistent band migration and intensity. All prestained protein ladders are compatible for use in western blotting.
On this page:
- Prestained broad and high molecular weight protein ladders
- Protein ladders for western blotting and fluorescence western blotting
- Unstained protein ladders
- Protein ladders for IEF, native PAGE and specialty applications
- Ordering information
- Compare all Protein standards and molecular weight ladders
Prestained broad and high molecular weight protein ladders
Prestained molecular weight standards are ideal to monitor the progression of the gel electrophoresis run and to estimate protein transfer efficiency. Available in blue and multicolor options, they are compatible with all Invitrogen SDS-PAGE gels. The combination of high molecular weight range protein ladders and NuPAGE Tris-Acetate protein gels is recommended for optimal separation and detection of high molecular weight proteins.
| Product description | PageRuler Plus Prestained Protein Ladder
Request sample | Spectra Multicolor Broad Range Protein Ladder Click image to enlarge | HiMark Prestained Protein Standard Click image to enlarge | SeeBlue Pre-Stained Standard Click image to enlarge |
|---|---|---|---|---|
| Choose for | Best multicolor prestained ladder for routine applications | 4 colors for improved visualization during separation and transfer | Analysis of high molecular weight proteins | Visible monitoring of gel separation and transfer efficiency |
| Range (kDa) | 10–250 | 10–260 | 31–460 | 3–200 |
| Number of bands | 9 | 10 | 9 | 9 |
| Recommended gel | All SDS-PAGE gels | All SDS-PAGE gels | NuPAGE Tris-acetate | All SDS-PAGE gels |
| Recommended Volume per well for 1.0 mm gel (μL) | 5 µL for electrophoresis 5 µL for blotting | 10 µL for electrophoresis 10 µL for blotting | 10 µL for electrophoresis 10 µL for blotting | 10 µL for electrophoresis 3 µL for blotting |
| Visualization method | Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) | Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) | Colorimetric | Colorimetric |
| Cat. No. | 26619 (2 x 250 µL), 26620 (10 x 250 µL) | 26634(2 x 250 µL),26623 (10 x 250 µL) | LC5699(250 µL) | LC5625(2 x 250 µL) |
Protein ladders for western blotting and fluorescent western blotting
Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Western blotting ladders provide a useful positive control to evaluate the performance of the detection antibody. Direct visualization of unstained ladders can also be achieved through standard staining protocols (e.g., Coomassie stains).
| Product description | iBright Prestained Protein Ladder Click image to enlarge Request Sample | MagicMark XP Western Protein Standard Click image to enlarge |
|---|---|---|
| Choose for | Exceptional versatility for visible, IgG or fluorescent western blot detection | IgG binding for direct visualization during detection of western blot |
| Range (kDa) | 11–250 | 20–220 |
| Number of bands | 12 | 9 |
| Recommended gel | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm gel (μL) | 1–3 | 5–10 |
| Visualization method | Colorimetric, NIR fluorescence (700 nm channel), user defined detection system (2 bands) | User defined detection system (not recommended for RGB fluorescence systems) |
| Properties | IgG binding sites on 2 bands (80 and 30 kDa), 10 fluorescent, blue-stained bands | IgG binding sites on all bands |
| Cat. No. | LC5615(2 x 250 µL) | LC5602(250 µL) LC5603(50 µL) |
Unstained protein ladders
Unstained protein ladders are useful when performing precise molecular weight determination of the target protein. Unstained protein standards are not labeled with dyes that can alter the apparent molecular weight of the ladder in the gel. They can be visualized by direct staining with Coomassie or other protein stains. PageRuler Unstained Protein Standards contain Strep-tagTMII sequence and can be immunodetected on the western blot.
| Product description | PageRuler Unstained Protein Ladder
| PageRuler Unstained Broad Range Protein Ladder Click image to enlarge | HiMark Unstained Protein Standard
|
|---|---|---|---|
| Choose for | 14 bands for superior accuracy | Accurate estimation across a broader range | Analysis of high molecular weight proteins |
| Range (kDa) | 10–200 | 5–250 | 40–500 |
| Number of Bands | 14 | 11 | 9 |
| Recommended gel | All SDS-PAGE gels | All SDS-PAGE gels | NuPAGE Tris- Acetate |
| Recommended Volume per well for 1.0 mm gel (μL) | 5 | 5 | 5 |
| Visualization method | stain of choice | stain of choice | stain of choice |
| Properties | Tagged—proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence | Tagged— proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence | Direct visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) |
| Cat. No. | 26614(2 x 250 µL) | 26630(2 x 250 µL) | LC5688(250 µL) |
Protein ladders for IEF, native PAGE and specialty applications
A selection of protein ladders engineered to provide a reliable reference for protein size determination in native PAGE, pI calibration, detection of His-Tagged, phosphorylated, or glycosylated proteins.
| IEF Marker 3-10
| NativeMark Unstained Protein Standard
| E-PAGE SeeBlue Pre-stained Standard
| BenchMark His-tagged Protein Standard
| Peppermint Stick Phosphoprotein Molecular Weight Standards
| CandyCane Glycoprotein Molecular Weight Standards
|
| No. of bands | |||||
| 13 | 8 | 5 | 10 | 6 | 8 |
| Band weights | |||||
| ~10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5 | ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa | ~261, 173, 97, 42, 21 kDa | ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDa | ~116, 66, 45, 23.6, 18, 14.4 kDa | ~180, 97, 82, 66, 42, 29, 18, 14 kDa |
| Colors | |||||
| Unstained | Unstained | Blue, orange, pink | Unstained | Unstained | Unstained |
| Visualization | |||||
| Colorimetric | Colorimetric | Colorimetric | Each protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel Stain | Colorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stains | Colorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits |
| Recommended gel | |||||
| IEF gels | Native-page gels | E-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels | All SDS-PAGE gels |
| Recommended volume per well for 1.0 mm mini gel | |||||
| 5 μL | 5 μL | 10 μL for electrophoresis, 5 μL for blotting | 5 μL for electrophoresis, 2.5–5 μL for blotting | 1 μL | 0.5 μL |
| Cat. No. | |||||
| 3921201 (500 µL) | LC0725 (5 x 50 µL) | LC5700 (500 µL) | LC5606 (125 µL) | P33350 (400 µL) P27167 (40 µL) | C21852 (400 µL) |
Ordering information
Prestained protein ladders
Western blot and fluorescent protein ladders
Unstained protein ladders
IEF and specialty protein ladders
Compare all protein standards and ladders
| Range kDa | No. of Bands | Recommended gel | Visible | Fluorescence | Western detection | ||
|---|---|---|---|---|---|---|---|
| NIR | RGB | ||||||
| Prestained Protein Ladders | |||||||
| PageRuler Plus Prestained Protein Ladder | 10–250 | 9 | all SDS-PAGE gels | Blue, Orange, Green | |||
| PageRuler Prestained Protein Ladder | 10–180 | 10 | all SDS-PAGE gels | Blue, Orange, Green | |||
| Spectra Multicolor Broad Range Protein Ladder | 10–260 | 10 | all SDS-PAGE gels | Blue, Orange, Green, Pink | |||
| SeeBlue Plus2 Pre-stained Protein Standard | 3–200 | 10 | all SDS-PAGE gels | Blue, Pink, Orange | |||
| Novex Sharp Pre-Stained Protein Standard | 3.5–260 | 12 | all SDS-PAGE gels | Blue, Pink, Orange | |||
| BenchMark Pre-stained Protein Ladder | 6–180 | 10 | all SDS-PAGE gels | Blue, Pink | |||
| HiMark Pre-stained Protein Standard | 30–460 | 9 | NuPAGE Tris-Acetate | Blue, Pink | |||
| Spectra Multicolor High Range Protein Ladder | 40–300 | 8 | NuPAGE Tris-Acetate | Blue, Orange, Green | |||
| Spectra Multicolor Low Range Protein Ladder | 1.7–40 | 6 | Novex Tricine | Blue, Orange, Green | |||
| SeeBlue Pre-Stained Standard | 3–200 | 9 | all SDS-PAGE gels | Blue | |||
| iBright Prestained Protein Ladder | 11–250 | 12 | all SDS-PAGE gels | Blue | IgG binding sites | ||
| PageRuler Prestained NIR Protein Ladder | 11–250 | 10 | all SDS-PAGE gels | Blue | |||
| Unstained Protein Ladders | |||||||
| MagicMark XP Western Protein Standard | 20–220 | 9 | all SDS-PAGE gels | stain of choice | IgG binding sites | ||
| PageRuler Unstained Protein Ladder | 10–200 | 14 | all SDS-PAGE gels | stain of choice | Strep-tag™ II sequence | ||
| PageRuler Unstained Broad Range Protein Ladder | 5–250 | 11 | all SDS-PAGE gels | stain of choice | Strep-tag™ II sequence | ||
| PageRuler Unstained High Range Protein Ladder | 60–250 | 8 | NuPAGE Tris-Acetate | stain of choice | Strep-tag™ II sequence | ||
| Mark12 Unstained Standard | 2.5–200 | 12 | all SDS-PAGE gels | stain of choice | |||
| BenchMark Protein Ladder | 10–220 | 15 | all SDS-PAGE gels | stain of choice | |||
| BenchMark Fluorescent Protein Standard | 11–155 | 7 | all SDS-PAGE gels | stain of choice | |||
| Novex Sharp Unstained Protein Standard | 3.5–260 | 12 | all SDS-PAGE gels | stain of choice | |||
| SuperSignal Molecular Weight Protein Ladder | 20–150 | 8 | all SDS-PAGE gels | stain of choice | IgG binding sites | ||
| SuperSignal Enhanced Molecular Weight Protein Ladder | 20–150 | 8 | all SDS-PAGE gels | stain of choice | IgG binding sites | ||
| Pierce Unstained Protein MW Marker | 14–116 | 7 | all SDS-PAGE gels | stain of choice | |||
| HiMark Unstained Protein Standard | 40–500 | 9 | NuPAGE Tris-Acetate | stain of choice | |||
| PageRuler Unstained Low Range Protein Ladder | 3.4–100 | 8 | Novex Tricine | stain of choice | Strep-tag™ II sequence | ||
Resources
Download: Protein Gel Guide
Download: Protein gel electrophoresis technical handbook
Download: Western Detection Workflow brochure
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